Abstract: By means of literature review, questionnaire, interview, instruction experiment and statistics, the study proposed the diversified teaching mode based on diversified instruction concept in the three aspects of teaching content, method and evaluation, and implemented the experimental instruction among 2011 Physical Education majors from Shanghai University of Sport. It is found that diversified teaching mode can improve the students' motivation, interest, tenacity and emotion in the process of instruction from the perspective of non-intellectual factors; and can promote the students' comprehension and understanding of the significance and core content of Pedagogy curriculum so as to enhance their competence in educational theory, promote their practical skills and increase their scores from the perspective of intellectual factors. The findings indicate that the diversified teaching mode has achieved a good effect.